拟南芥抗病相关基因AtBT4的原核表达分析

In order to obtain the purified protein AtBT4, prokaryotic expression vector of resistance-related gene AtBT4 from Arabidopsis thaliana was constructed and expressed efficiently in this study. The CDS of AtBT4 was amplified by RT-PCR technology using the cDNA of the 4-week-old Col-0 seedlings and cloned into a prokaryotic expression vector pGEX-4T-1. Restriction enzyme digestion and sequencing showed that the recombinant vector pGEX-AtBT4-GST was successfully constructed and transformed into E. coli BL21 cell. The results of SDS-PAGE assay indicated that pGEX-AtBT4-GST was successfully expressed in E. coli BL21 strain, with the molecular weight 69 kDa. These results would provide basis for screening interacting proteins of AtBT4 and regulation mechanism in Arabidopsis resistance.