上调Foxp3基因表达抑制上皮卵巢癌细胞株SKOV3增殖的研究

Objective: To construct the eukaryotic expressing vector for Forkhead helix transcription factor (Foxp3) and transfect it to human epithelial ovarian cancer cells for stable expression of Foxp3 for investigating the role and mechanism of Foxp3 to inhibit tumor cells activities. Methods: Eukaryotic expressing vector pEF-1α-Foxp3 was constructed and transfected into SKOV3 cells by lipofectamine protocols. After G418 selection, the cells expressing Foxp3 stably （SKOV3/pEF1-α-Foxp3）were obtained. Multiple cellular and molecular approaches such as cell growth assay, western blot were used to detect the consequences of up-regulation Foxp3 in SKOV3 cells. Results: The eukaryotic expressing vector pEF1-α-Foxp3 was constructed and Foxp3 was expressed in SKOV3 cells. Up-regulation of Foxp3 inhibited cells growth. Furthermore, up-regulation of Foxp3 inhibited activation of mTOR signaling, which play important role in cell growth. Conclusions: Foxp3 plays a suppressive role in epithelial ovarian cancer partially through negatively regulating mTOR signaling. Up-regulation of Foxp3 could be a novel approach for the inhibition of epithelial ovarian cancer progression.