腺病毒技术封闭smad4基因表达对人原代血管内皮细胞增殖和迁移的影响

Background and Objective：To investigate the effect of silencing smad4 gene expression by using adenovirus on proliferation and migration of HVEC cell. Methods : Adenovirus targeting smad4 gene were designed. Adv-smad4 was transfected into HVEC cells. The alteration of smad4 expression was examined by Western blot. The proliferation capacity of HVEC cells was measured by CFSE-flow cytometry. The monostratal wounding healing was used to determine the migration capacity of HVEC cells. Results: Adv-smad4 was successfully transfected into HVEC cells. Transfection of Adv-smad4 significantly knocked down the smad4 protein expression. The proliferation and migration capacity of HVEC cells transfected with Adv-smad4 was significantly enhanced compared with that of control group. Conclusions:The specific smad4-targeted adenovirus can efficiently reduce endogenous smad4 gene expression in HVEC cells. Selectively smad4 gene silencing can promote the proliferation and migration of HVEC cells.