氯化镉致心肌细胞线粒体的损伤

Objective：To study damage effects of mitochondria of cardiomyocytes induced by CdCl2. Methods：Incubate cardiomyocytes of rats with the primary cell culture method. Examine survival rate of myocytes with MTT assay, and detect avtivities of ATP enzyme and LDH with biochemical method, MMP with FCM, MPT with spectrophotometry. Results: In exposure groups, 5µmol/L CdCl2 could decrease survival rate of cardiomyocytes, activities of ATP enzyme and LDH, membrane potential of mitochondria, and membrane permeability of mitochondria was changed induced by CdCl2. There were statistically significant differences between control and exposure groups (P＜0.05). Conclusions: CdCl2 could induce damage of mitochondria of cardiomyocytes, and there possibly exits a dose dependent relationship.