miR-126在高糖诱导的脉络膜视网膜内皮细胞应激中的作用研究

Objective: miR-126, the miRNA considered to be specially expressed in endothelial cells and hematopoietic progenitor cells, is strongly associated with angiogenesis. The purpose is to evaluate the role of miR-126 in high glucose-induced angiogenesis and the possible mechanisms. Methods: The expression of miR-126 was detected in high glucose-treated RF/6A cells and diabetic retinas using real-time PCR. The miR-126 was up-regulated by transfecting miR-126-mimics into RF/6A cells. The protein level of vascular endothelial growth factor (VEGF) was assessed by immunoblotting. Results: A significantly decreased expression of miR-126 was found in high glucose-treated RF/6A cells compared with control. The expression of miR-126 was also reduced in the retina tissue of streptozotocin-induced diabetic rats. The expression of VEGF protein was increased in high glucose-induced RF/6A cells. The VEGF protein level was sharply decreased in high glucose-induced RF/6A cells pretreated with miR-126-mimics. Conclusions: miR-126 is down-regulated under high glucose condition both in vitro and in vivo and may halt the high glucose-induce neovascularization by inhibiting the expression of VEGF.