The rust fungus Melampsora larici-populina expresses a conserved genetic program and distinct sets of secreted protein genes during infection of its two host plants, larch and poplar
Lorrain C¨|cile 1P¨|trowski J¨|r¨|my 1Petre Benjamin 2Hacquard St¨|phane 1Delaruelle Christine 1Frey Pascal 1Hecker Arnaud 1Duplessis S¨|bastien 1Marchal Cl¨|mence1
作者信息
- 1. INRA/Universit¨| de Lorraine
- 2. INRA/Universit¨| de Lorraine||The Sainsbury Laboratory, Norwich Research Park
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Abstract
Summary
Mechanims required for broad spectrum or specific host colonization of plant parasites are poorly understood. As a perfect illustration, heteroecious rust fungi require two alternate host plants to complete their life cycle. Melampsora larici-populina infects two taxonomically unrelated plants, larch on which sexual reproduction is achieved and poplar on which clonal multiplication occurs leading to severe epidemics in plantations. High-depth RNA sequencing was applied to three key developmental stages of M. larici-populina infection on larch: basidia, pycnia and aecia. Comparative transcriptomics of infection on poplar and larch hosts was performed using available expression data. Secreted protein was the only significantly over-represented category among differentially expressed M. larici-populina genes in basidia, pycnia and aecia compared together, highlighting their probable involvement in the infection process. Comparison of fungal transcriptomes in larch and poplar revealed a majority of rust genes commonly expressed on the two hosts and a fraction exhibiting a host-specific expression. More particularly, gene families encoding small secreted proteins presented striking expression profiles that highlight probable candidate effectors specialized on each host. Our results bring valuable new information about the biological cycle of rust fungi and identify genes that may contribute to host specificity.Key words
Pucciniales/Rust fungi/Heteroecism/Basidiospores/Pycniospores/Aeciospores/comparative transcriptomics/effectors引用本文复制引用
Lorrain C¨|cile,P¨|trowski J¨|r¨|my,Petre Benjamin,Hacquard St¨|phane,Delaruelle Christine,Frey Pascal,Hecker Arnaud,Duplessis S¨|bastien,Marchal Cl¨|mence.The rust fungus Melampsora larici-populina expresses a conserved genetic program and distinct sets of secreted protein genes during infection of its two host plants, larch and poplar[EB/OL].(2025-03-28)[2026-05-01].https://www.biorxiv.org/content/10.1101/229971.学科分类
植物学/遗传学/分子生物学
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