花背蟾蜍Paired box 6 variant基因与Paired box 6基因的功能异同与具体功能位点的探究

Paired box 6 (Pax6) is a highly conserved genes, it plays an important role in the process of regulating the vertebrate eye development. Paired box 6 variant (Pax6 variant, VP) is a Bufo raddei Pax6 gene families of homologous genes in the body. This experiment mainly focus on pax6 of Xenopus to VP of Bufo raddei, the similarities and differences between the two kind of cells on cell proliferation, cycle regulation and the influence of cell fate determination, as well as the change of the related sites impact on the cell proliferation and its downstream, in order to predict which specific sites plays main function. Phosphorylation sites prediction of PAX6 and VP protein and sequence alignment of them shows that the difference between them are in the structure of PST domain, and the phosphorylation sites or sites can be recognized on the phosphorylation of kinases reduces significantly in the VP. For its different phosphorylation site structure，we design point mutation plasmid and do the related experiment, we speculate that the 373 phosphorylation sites of the gene plays as the leading factor, the other phosphorylation site has the corresponding effect.