副溶血性弧菌外膜亚蛋白质组图谱的建立及其意义
he establishment and significance of V. parahaemolyticus sub-outer membrane proteome
副溶血性弧菌(V. parahaemolyticus)具有嗜盐性，是引起海水养殖动物病害和海产品食物中毒的常见重要病原菌。本文采用月桂酰基氨酸钠二步法，提取了副溶血性弧菌的外膜蛋白,首次建立了外膜亚蛋白质组表达图谱,鉴定了29个外膜蛋白,占所鉴定蛋白的78.3%。这些研究结果对副溶血性弧菌外膜蛋白功能的研究具有积极作用。此外，对外膜蛋白组学研究的有关技术问题进行了相关探讨。
V. parahaemolyticus, a halophilic salt-requiring bacterium, is a important Gram-negative pathogen that cause great losses in aquaculture and sea food-borne diseases in human. In the present study, sub-outer membrane proteome of this bacterium was established using two-steps of sarcosine-insoluble fraction. Twenty-nine outer membrane proteins was identified, being 78.3% of the identified proteins. These results will be helpful in study of roles of V. parahaemolyticus outer membrane proteins. In addition, techniques on outer membrane proteome was addressed.
王三英、徐常新、李惠、彭宣宪
微生物学生物科学研究方法、生物科学研究技术生物化学
副溶血性弧菌外膜蛋白蛋白质组学
V. parahaemolyticusouter membrane proteinproteomics
王三英,徐常新,李惠,彭宣宪.副溶血性弧菌外膜亚蛋白质组图谱的建立及其意义[EB/OL].(2010-01-14)[2025-04-27].http://www.paper.edu.cn/releasepaper/content/201001-562.点此复制
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