BSA固定化的PS纳米微球涂层及其开管毛细管电色谱法分离手性色氨酸

In this work, polystyrene (PS) nanoparticles coated by bovine serum albumin (BSA), denoted as PS/BSA，were fabricated and chemically immobilized for the first time onto a capillary innerwall, serving as the stationary phase for open tubular capillary electrochromatography. The prepared PS/BSA nanoparticles as well as its coating onto the capillary were characterized in detail. The capillary column coated by PS/BSA nanoparticles was used for chiral separaiton of D,L-tryptophan. As a result, the resolution was significantly improved by the nanoparticle-immobilized capillary, compared with monolayer BSA-modified capillary. The resolution was increased with the decrease of the diameter of the nanoparticle. Besides, run-to-run and column-to-column reproducibility of the PS/BSA-coated column in the chiral separation were systematically introduced.