烟草水杨酸受体NtNPR1a在烟草抗黑胫病中的功能研究
Functional of tobacco salicylic acid receptor NtNPR1a in tobacco resistance to phytophthora nicotiance
黑胫病是烟草疫霉（phytophthora nicotianae）侵染导致的一种严重危害烟草生产的土传性疾病，给烟草种植业造成严重的经济损失。水杨酸（salicylic acid，SA）能调节植物对抗病原体的反应，拟南芥中AtNPR1、AtNPR3/4与水杨酸结合，参与水杨酸信号转导，在水杨酸参与的植物系统获得抗性（SAR）反应中起着重要的传递作用。本文筛选出四条与AtNPR1、AtNPR3/4同源的烟草序列，命名为NtNPR1a、NtNPR1b、NtNPR3a、NtNPR3b，通过生物信息学方法分析其结构及理化性质，通过实时荧光定量PCR方法分析其表达模式，通过农杆菌介导的瞬时表达，发现NtNPR1a基因基因瞬时表达接菌后的病斑面积极显著小于空载对照的病斑面积，这表明NtNPR1a基因在烟草对抗烟草黑胫病的过程中表现出一定抗性。该研究将为烟草黑胫病的防治提供新的基因资源，筛选出可用于农作物基因工程改良的靶标基因，具有重要的理论意义和价值。
植物保护农业科学研究植物学
植物保护水杨酸NPRs烟草黑胫病烟草疫霉
汪兴静,江兴月,段宇飞,李赜宣,张建奎.烟草水杨酸受体NtNPR1a在烟草抗黑胫病中的功能研究[EB/OL].(2024-05-17)[2025-10-30].http://www.paper.edu.cn/releasepaper/content/202405-99.点此复制
Black shank disease is a serious soil borne disease that poses a serious threat to tobacco production and causes serious economic losses to the tobacco planting industry due to the infection of Phytophthora nicotianae. Salicylic acid (SA) can regulate the plant\'s response to pathogens. In Arabidopsis, AtNPR1 and AtNPR3/4 can bind with salicylic acid and participate in salicylic acid signal transduction, playing an important role in the transmission of salicylic acid induced resistance (SAR) in the plant system. This article screened four tobacco sequences homologous to AtNPR1 and AtNPR3/4, named NtNPR1a, NtNPR1b, NtNPR3a, and NtNPR3b. Their structures and physicochemical properties were analyzed using bioinformatics methods, and their expression patterns were analyzed using real-time fluorescence quantitative PCR. Through Agrobacterium mediated transient expression, it was found that the lesion area of NtNPR1a gene after transient expression was significantly smaller than that of the empty control. This indicates that NtNPR1a gene exhibits certain resistance in tobacco against tobacco black shank. This study will provide new genetic resources for the prevention and control of tobacco black shank, and screen target genes that can be used for crop genetic engineering improvement, which has important theoretical significance and value.
plant protectionsalicylic acidNPRsTobaccoblack shankphytophthora nicotianaekey
展开英文信息
评论