椭圆叶花锚种子萌发与其内源激素含量变化的关系
The relationship between germination and endogenous hormones content change in the seeds of Halenia elliptica
本研究以青藏高原东北缘高寒草甸常见植物椭圆叶花锚种子为实验材料,测定了椭圆叶花锚种子在不同的储藏条件及时间内,种子的萌发率及种子中内源激素玉米素(ZT)、赤霉素(GAs)、6-苄氨基嘌呤(6-BA)、生长素(IAA)和脱落酸(ABA)含量,以分析椭圆叶花锚种子萌发与其内源激素含量变化的关系,为椭圆叶花锚的栽培提供理论基础。结果表明:(1)储藏条件和时间对椭圆叶花锚种子的萌发率均有极显著影响。种子萌发率在常温储藏条件下大约是冷藏条件下的4倍,说明常温条件有利于椭圆叶花锚的储藏。椭圆叶花锚种子的休眠可能属于季节性休眠,这是长期适应环境的结果。(2)不同储藏条件下,椭圆叶花锚种子中内源激素含量随储藏时间变化差异显著。在常温储藏条件下,储藏时间对内源激素(ZT、GAs、6-BA、IAA、ABA)的绝对含量及各生长促进物与抑制物的比值(ZT/ABA、GAs/ABA、6-BA/ABA)均有较显著的影响,但对IAA/ABA无显著影响。在冷藏条件下,储藏时间仅对GAs、6-BA和IAA的绝对含量有较显著的影响。(3)对于椭圆叶花锚种子来说,赤霉素(GAs)与萌发率呈显著正相关,生长素(IAA)与萌发率呈显著负相关,其它内源激素(ZT、6-BA、ABA)及各促进物与抑制物的比值与萌发率均无显著地相关性。
In this study, seeds of Halenia elliptica which is widely spread in the northeast Qinghai-Tibet plateau were used to test the dynamic of endogenous hormones( zeatin, gibberellin, 6-Benzylaminopurine, auxin and abscisic acid ) content and germination in different storage conditions and time,to analyze the relationship between germination and endogenous hormones content change in the seeds of Halenia elliptica,to provide reference information for Halenia elliptica cultivation.The results showed that:(1) The storage time and storage conditions have significant influence on the percent germination.The percent germination of seeds under room temperature was about 4 times higher than that under cold storage,stating that room temperature was more conductive to seed conservation.The dormancy of the seeds of elliptical anchor may belong to the seasonal dormancy,this is the result of long time to adapt to the environment.(2) The endogenous hormone contents of seeds were varying with storage time in different conditions. Under room temperature, the effects of storage time on the absolute contents of endogenous hormone(ZT、GAs、6-BA、IAA、ABA)and the ratio of promoters to inhibitors(ZT/ABA、GAs/ABA、6-BA/ABA)were significant,while had no significient effect on IAA/ABA.Under cold storage, the effects of storage time on the content of GAs、6 -BA and IAA were significant.(3) Percent seed germination of Halenia elliptica was positively correlated with the content of GAs and negatively with the content of IAA, while other endogenous hormones (ZT, 6-BA, ABA) and the ratio of promoters to inhibitors have no significant effect on seed germination.
张雁明、徐当会、葛文静、夏玉斌、卜海燕、刘坤、王顺霞、齐威
农业科学研究植物学
椭圆叶花锚种子萌发储藏时间储藏条件内源激素
Halenia ellipticaseed germinationstorage conditionsstorage timeendogenous hormones
张雁明,徐当会,葛文静,夏玉斌,卜海燕,刘坤,王顺霞,齐威.椭圆叶花锚种子萌发与其内源激素含量变化的关系[EB/OL].(2017-03-27)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201703-358.点此复制
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