玻璃体视网膜增殖膜中survivin 基因的表达研究

PURPOSE Proliferative diabetic retinopathy (PDR), Proliferative vitreoretinopathy(PVR) and idiopathic macular preretinal membrane(IMPRM) results from the formation of preretinal membranes (PRMs) in the posterior fundus that can lead to a severe decrease of vision. Survivin (SVV) is a protein that is highly expressed in the cells of tumors, but whether it plays a role in PRMs is unknown. The purpose of this study was to determine whether SVV is associated with the formation of PRMs. METHODS PRMs were obtained during vitrectomy from patients with PDR, PVR and IMPRM. RT-PCR was performed to determine the level of expression of the mRNA of SVV. Immunofluorescence analyses were performed to determine the sites of SVV in the PRMs. RESULTS The level of the mRNA of SVV was high in 6 of 6 PRMs from patients with PDR, 5 of 5 PRMs from patients with PVR and 6 of 6 PRMs from patients with IMPRM. Immunofluorescence analysis showed the colocalizations of SVV and CD31, an endothelial cell marker, SVV and cytokeratin PAN, a retinal pigment epithelial cell marker, SVV and Glial Fibrillary Acidic Protein(GFAP), a gial cell marker, SVV and vimentin, a fibroblast cell marker, SVV and fibronectin(FN), laminin(LN), two members of the extracellular matrix proteins in most of the cells in the PRMs from PDR. Immunofluorescence analysis showed the colocalizations of SVV and GFAP, SVV and vimentin, SVV and fibronectin, laminin in most of the cells in the PRMs from PVR. Immunofluorescence analysis showed the colocalizations of SVV and GFAP, SVV and vimentin in most of the cells in the PRMs from IMPRM. CONCLUSIONS This study indicates that SVV may play a significant role in the proliferation and maintenance of most cells in the PRMs. If correct, SVV may be a molecular target for new diagnostic and therapeutic strategies for PDR, PVR and IMPRM.