L3促进T淋巴细胞穿过脑微血管内皮细胞单层

It has been showed that T lymphocytes might participate in the inflammation process in Alzheimer’s disease (AD), however, it is unclear how circulating T cells cross the blood-brain barrier (BBB). We have showed the stronger ability to migrate through human brain microvascular endothelial cells (HBMECs) and a significantly higher macrophage inflammatory protein-1α (MIP-1α, CCL3) expression in peripheral T lymphocytes of AD patients than age-matched healthy subjects. In order to explore the mechanism of the transendothelial migration of T lymphocytes further, rhMIP-1α or 6T-CEM, the cell line of human lymphoblastic leukemia which highly expressed MIP-1α, were incubated with HBMEC monolayer separately. It was showed that rhMIP-1α could enhance the migration of 6T-CEM cells through HBMEC monolayer and disrupt the tight junction of HBMEC. The expressions of CC chemokine receptor 5 (CCR5) on HBMECs which incubated with rhMIP-1α or 6T-CEM cells were detected. These data suggested that MIP-1α might promote the transendothelial migration of T lymphocytes by interacting with CCR5 on HBMEC monolayer.