壳寡糖进入细胞的机制研究

Oligosaccharides has varieties of biological activities, such as anti-infective; induce apoptosis in cancer cells; regulate immunity. But the mechanism of oligochitosan enter the cells is unclear, we oligochitosan fluorescently labeled observe the microscopic mechanism oligochitosan into cells. Experimental results show that chitosan oligosaccharide into the cell caused by membrane fluidity and temperature , when elevated temperatures makes oligochitosan enter the cell content increased, A23187 can significantly accelerate the oligochitosan into the cell by increase membrane fluidity. In addition, we prove that A23187 can facilitate membrane fluidity due to an increase in the plasma membrane phosphatidylserine concentration.