Cell cycle-dependent mRNA localization in P-bodies
Cell cycle-dependent mRNA localization in P-bodies
Abstract Understanding the dynamics of RNA targeting to membraneless organelles is essential to disentangle their functions. Here, we investigate how P-bodies (PBs) evolve during cell cycle progression. PB purification at different cell cycle phases uncovers widespread changes in their RNA content. Importantly, these changes are partly uncoupled from cell cycle-dependent changes in RNA expression. Single molecule FISH shows various mRNA localization patterns in PBs peaking in G1, S, or G2, with multiple examples illustrating the timely capture of mRNAs in PBs when their encoded protein becomes dispensable. Cyclic mRNA localization in PBs does not directly reflect their non-polysomal pool. While mRNA localization in PBs is tightly linked to their GC content at all cell cycle phases, PB mRNAs are specifically longer in G1. Altogether, our study supports a model where PBs are more than a default location for excess untranslated mRNAs and suggests an RNA-mediated seeding mechanism for post-mitotic PB reassembly. HighlightsThe RNA content of PBs is dynamics during cell cycle progressionCyclic mRNA accumulation in PBs is in part uncoupled from mRNA expression in the cytoplasmsmFISH highlights various patterns of cyclic mRNA accumulation in PBs, particularly in G1PBs do not buffer excess non-polysomal mRNAsPB mRNAs have particularly long CDSs in G1 but not G2, while they are strikingly AU-rich all along the cell cycle
Ernoult-Lange Mich¨¨le、Courel Ma?t¨|、Chevreux Guillaume、Gautheret Daniel、Pourcelot Oriane、Blugeon Corinne、Godebert Annie Munier、Slimani Floric、Coleno Emeline、Walter Thomas、B¨|nard Marianne、Kress Michel、Safieddine Adham、Weil Dominique、Vinit Angelique、Benassy Marie-No?lle、Imbert Arthur、Laine Antoine、Bertrand Edouard、Bonte Thomas
Sorbonne Universit¨|, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du D¨|veloppementSorbonne Universit¨|, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du D¨|veloppementUniversit¨| Paris Cit¨|, CNRS, Institut Jacques MonodInstitute for Integrative Biology of the Cell, UMR 9198, CEA, CNRS, Universit¨| Paris-SaclayInstitut de G¨|n¨|tique Humaine, University of Montpellier, CNRSG¨|nomique ENS, Institut de Biologie de l?ˉENS (IBENS), D¨|partement de biologie, ¨|cole normale sup¨|rieure, CNRS, INSERM, Universit¨| PSLSorbonne University, CISA flow cytometry facility, Research Center Saint-Antoine (CRSA), UMRS 938Institut de G¨|n¨|tique Humaine, University of Montpellier, CNRSInstitut de G¨|n¨|tique Humaine, University of Montpellier, CNRSCentre for Computational Biology (CBIO), Mines Paris, PSL UniversitySorbonne Universit¨|, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du D¨|veloppementSorbonne Universit¨|, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du D¨|veloppementSorbonne Universit¨|, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du D¨|veloppementSorbonne Universit¨|, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du D¨|veloppementSorbonne University, CISA flow cytometry facility, Research Center Saint-Antoine (CRSA), UMRS 938Sorbonne Universit¨|, CNRS, Institut de Biologie Paris Seine (IBPS), Laboratoire de Biologie du D¨|veloppementCentre for Computational Biology (CBIO), Mines Paris, PSL UniversityInstitute for Integrative Biology of the Cell, UMR 9198, CEA, CNRS, Universit¨| Paris-SaclayInstitut de G¨|n¨|tique Humaine, University of Montpellier, CNRSCentre for Computational Biology (CBIO), Mines Paris, PSL University
细胞生物学分子生物学
P-bodiesRNA localizationRNA imagingcell cycle
Ernoult-Lange Mich¨¨le,Courel Ma?t¨|,Chevreux Guillaume,Gautheret Daniel,Pourcelot Oriane,Blugeon Corinne,Godebert Annie Munier,Slimani Floric,Coleno Emeline,Walter Thomas,B¨|nard Marianne,Kress Michel,Safieddine Adham,Weil Dominique,Vinit Angelique,Benassy Marie-No?lle,Imbert Arthur,Laine Antoine,Bertrand Edouard,Bonte Thomas.Cell cycle-dependent mRNA localization in P-bodies[EB/OL].(2025-03-28)[2025-05-24].https://www.biorxiv.org/content/10.1101/2024.04.16.589748.点此复制
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