家蚕hsp23.7启动子瞬时表达载体的构建及活性检测

n order to verify the activity of the heat shock protein gene promoter from silkworm (hsp23.7) , the hsp23.7 promoter was amplified and cloned into the vector pSK with red fluorescent protein gene (DsRed ),then transient expression vector pSK- hsp23.7-DsRed-PolyA was constructded ;. It was transfected into silkworm BmN cells and silkworm tissues.The result showed that after the hsp23.7 promoter was treated using heat shock,it could induce DsRed gene expression in BmN cells and silkworm tissues. Consequently, the hsp23.7 promoter sequence have the heat shock protein promoter activity;The sequence analysis indicated that secondary structure of hsp23.7 promoter sequence forms a complicated stem-loop structure which could be related with the effection of the promoter.