MA单独或与CA混合对雄性小鼠精子质量的影响

In order to study the effect of melamine alone or cyanuric acid mixture on the sperm quality of male mice in an acute experiment.42 male SPF KM mice were divided into 4 groups randomly.Melamine and cyanuric acid were mixed at the ratio of 1:1.Then the mice from three group were administered instagastrically with low dosage(30mg/kg.bw.d), moderate dosage (140mg/kg.bw.d) and high dosage(700mg/kg.bw.d), respectively for 3 days Simultaneously,the control mice were administered with equivalent corn oil. At 24h after the last administration,the blood was collected and then all the mice were sacrificed.Prepare the sperm suspension,count the quantity of the sperm,the sperm motility rate and the sperm abnormality rate.Detect the sperm DNA damage by single cell gel electrophoresis.The results showed that high doses of MA-CA can cause a significant decrease in the number of sperm in epididymis.MA and MA-CA in each dose group significantly reduced the living sperm rate, significantly increased the rate of sperm deformity, especially the head deformity rate, and showed a dose effect.High dose of MA and MA-CA caused significant damage to sperm DNA.These results indicate that MA and MA-CA in acute test have obvious damage to sperm quality in male mice, and MA-CA is more serious damage.