不同基质上绿僵菌孢子萌发及附着孢观察

onidium germination and appressorium formation are key steps in the process of infecting insect hosts by entomopathogenic fungi. Growth media with four different nutrition levels combined with various cultivation intervals were utilized to study the effect on conidial germination and appressorial development, as well as properties of germ tube and appressorium of Metarhizium anisopliae, CQMa102, when applied on glass surface and locust wing. Results showed that nutrition level of the growth media and cultivation interval significantly affected conidial germination and appressorial formation. Nutrient-rich media enhanced conidial germination but negatively impacted appresorial formation. Appressoria were formed mostly during the period of 24 - 48h post cultivation. Compared with glass surface, there were more appressoria produced on locust wing. Moreover, larger sized appressoria with germ tubes having shorter length, fewer branches, and fewer septa were found on locust wing than those on the glass surface.