新加良附方联合5-Fu对裸鼠胃癌移植瘤Survivin/Caspase-3表达的影响

Objective：To observe the effect of Xinjialiangfu (XJLF) Formula on human gastric cancer (SGC-7901) tumor growth in nude mice and its impact on Survivin and Caspase-3 expression in tumor tissue. Methods：The animal model of SGC-7901 xenografted nude mice was established, and all the mice were randomly divided into 6 groups; namely model control group, 5-Fu group, XJLF Formula high-dose group, XJLF Formula medium-dose group , XJLF Formula low-dose group and drug combination group (XJLF Formula medium-dose and 5-Fu). After 10 days of continuous treatment, tumor inhibition rate was calculated and the expression of Survivin and Caspase-3 was detected by immunohistochemistry techniques. Results: The tumor inhibition rate of drug combination group was 65.28%, which was significantly increased compared to the model control group (P<0.05), 5-Fu group (P<0.05) and XJLF high-dose group (P<0.05), respectively. Meanwhile, the Caspase-3 expression was significantly up-regulated and the Survivin expression was significantly down-regulated in drug combination group, compared to the model control group (P<0.05，P<0.01), 5-Fu group (P<0.05，P<0.05) and XJLF Formula high-dose group (P<0.05，P<0.05), respectively. Conclusion: The tumor inhibition rate of drug combination group is higher than XJLF Formula high-dose group and 5-Fu group. Also combination group has stronger effect compared to model control group in terms of down-regulation of Caspase-3 expression and up-regulation of Survivin expression, which indicates a benefit of XJLF and 5-Fu combination treatment.?????