均匀设计在对硫磷ELISA方法

Pesticide parathion was chosen as the object, the application of uniform design for the optimization of ELISA method was investigated and compared with the common optimization method. The common method for the optimization of ELISA showed the optimal conditions were 5% methanol, ionic strength 0.5 M and pH7.4. Then the ELISA method was set up. The IC50 and IC20 were 89.17 ng/mL and 12.49 ng/mL, respectively. On the other hand, the uniform design was introduced into the optimization of ELISA and the results were computed by statistical method. Then the forecasting model was brought out and the best conditions were concluded. The results showed the optimal conditions were 10% methanol, ionic strength 2 M and pH5. The IC50 and IC20 of the ELISA method were 9.96 ng/mL and 1.21 ng/mL, respectively. In addition, the two ELISAs with the different conditions which come from the two optimization methods were applied for the parathion residue determination in five kinds of soils. The results showed the application of uniform design for the optimization of ELISA method was more fast and easily, when compared with the common method. Moreover, the concluded conditions from the uniform design method could result in the ELISA method has a broad-specific character, and the method could be applied for pesticide residue determination in different samples with different matrix effects.