小鼠乳腺中Heregulin-α的信号转导通路

o determine the signal transduction pathway of Heregulin-α in mammary gland, the mammary gland of mouse at 8 days in pregnancy, 12 days in lactation and 6 days in involution were cultured in vitro. Each individual mammary gland was cultured in the media Waymouth′s MB752/1 with or without Heregulin-α. Following 24 hours of culture, each mammary gland was digested to obtain epithelial cells, which were lysed and the lysate was used for western blot analysis. Western blot results showed that Heregulin-α enhanced the phosphorylation of p44/42 MAPK, p38 MAPK and PKC in pregnancy; and, Heregulin-α promoted the phosphorylation of STAT5 and p38 MAPK, while it inhibited PKC phosphorylation in lactation; in involution, Heregulin-α induced STAT3 phosphorylation and inhibited PKC phosphorylation.