硫酸化茶藨子木层孔菌多糖的制备与活性研究

Objective To prepare sulfated polysaccharide from Phellinus ribis (PRP-S) and study their antitumor activities compared with Phellinus ribis polysaccharide (PRP) in vitro. Method PRP-S was prepared by chlorosulfonic acid- formamide method. The effects on human ovary cancer SKOV-3 cells were observed in vitro by MTT method. Result: The degrees of sulfate of three sulfated derivatives (PRP-S) were 1.48, 1.83 and 2.23, respectively. Two characteristic absorption bands (S=O and C-O-S) appeared in FT-IR spectra. PRP had no obvious influence on SKOV-3 cells, however PRP-S exhibited much stronger inhibition effects against SKOV-3 cell growth. Conclusion: PRP-S was obtained successfully without degradation of the polysaccharide. PRP-S showed significant inhibition effects on SKOV-3 cells in comparison with the native non-sulfated polysaccharide.