溴氰菊酯对飞蝗羧酸酯酶基因表达的影响

Objective: To analyze the mRNA expression patterns of carboxylesterase genes of L. migratoria to deltamethrin exposures. Methods: locusts exposed to different doses of deltamethrin and treated with deltamethrin at different exposure times were collected as templates, respectively. cDNAs were synthesized from total RNA of different templates using reverse transcriptase. Further, mRNA expression response of carboxylesterase genes of L. migratoria to deltamethrin exposures were confirmed by Real-time PCR. Results, exposures of deltamethrin at different doses for 12 h suppressed the mRNA expressions of most carboxylesterase genes. Except for LmCesA3 and LmCesE1, the transcript levels of most carboxylesterase genes were induced at early treated time and were suppressed at later treated time when the locusts were treated with deltamethrin at different exposure times. Conclusion: deltamethrin influenced the expression of carboxylesterase genes, ranging from induction to repression. The results will be helpful in providing strategies for insecticide resistance management of L. migratoria.