基于金胶的表面增强拉曼光谱（SERS）检测食源性致病菌

Forborne pathogens are very important threats to food safety, in which intestinal pathogens, nonspore-bearing , the whole body flagella or flagella , is a large class of high outbreaks of food contamination caused by not only hazardous to human health, but also not conducive to the food trade. Therefore, this paper studies the application of gold nanoparticle surface enhanced Raman spectroscopy (SERS) on detection four kinds of pathogenic bacteria: Shigella- Shigella dysenteriae, Shigella boydii，Shigella sonnei ; Salmonella - Salmonella typhimurium, Salmonella O7HZ10; Citrobacter - Citrobacter freundii and Enterobacter - Enterobacter sakazakii. At the same time, different batches of gold colloid and Enterobacter sakazakii repetitive verification SERS enhancement and stability. Second, it studied the impact of the SERS spectra of high pressure steam sterilization of pathogenic. Finally, seven kinds of pathogens by principal component analysis (Principal Component Analysis, PCA) and cluster analysis (Hierarchical Cluster Analysis HCA). Studies have shown that the SERS spectra of the seven kinds of bacteria than the literature and the attribution of a particular peak in the case of SERS good stability, the application of PCA and HCA to classify the more obvious distinction between each genus and each species. At the same time, before and after the autoclave sterilization SERS can be seen that the bacterial protein occurred degeneration.