Lewis y抗原对人卵巢癌细胞RMG-I-H部分耐药相关蛋白基因表达的影响

Objective To investigate the influence of Lewis y antigen on the gene expression of partial drug resistance associated proteins in human ovarian cancer cell line RMG-I-H. Methods RT-PCR was used to determine the gene expression of partial drug resistance associated proteins in RMG-I-H cell line transfected with α1, 2-fucosyltransferases gene and RMG-I cell line, as well as in RMG-I-H treated with or without anti-Lewis y monoclonal antibody. The immunocytochemical method was used to detect the expression of P-glycoprotein (P-gp) in RMG-I and RMG-I-H cell lines. RMG-I and RMG-I-H cells were transplanted into nude mice and the expression of P-gp in the tissues was measured by immunohistochemistry. Results The mRNA expression of multidrug resistance- associated protein-1(MRP-1), MRP-2, protein kinaseC-α(PKC-α), and topoismeraseⅠ(TopoⅠ)in RMG-I-H cells were higher than that in RMG-I cells. However, the mRNA expression of multidrug resistance 1 (MDR-1) was lower in RMG-I-H cells than that in RMG-I cells. The P-gp level increased in RMG-I-H cells compared with that in RMG-I cells both in vivo and in vitro. Expression of MDR-1, MRP-1, MRP-2, PKC-α, TopoⅠmRNA decreased by the time in RMG-I-H cells treated with anti-Lewis y monoclonal antibody. Additionally, MDR-1, MRP-1, MRP-2, PKC-α, TopoⅠmRNA expressions were lower in RMG-I-H cells treated with anti-Lewis y monoclonal antibody than the ones in the control group at 6 hour. Conclusion There is a close relationship between the Lewis y antigen on the human ovarian cancer cell surface and the regulation on the gene expression of partial drug resistance associated proteins.