芳香烃受体在双酚A所致斑马鱼胚胎发育毒性中的作用研究

[Objective] This research uses zebrafish as a model animal to study the role of AhR in the developmental toxicity of zebrafish embryos induced by BPA. [Methods] In this study, zebrafish embryos were randomly divided into 8 groups for exposure: DMSO control group, AhR inhibitor CH223191 (CH) control group, BPA exposure group (1 μM, 5 μM, 25 μM), CH+BPA (1 μM, 5 μM, 25 μM) groups. The exposure period was from 2hpf to 96hpf. The mortality, hatching rate, malformation rate, heart rate, body length and locomotor ability of zebrafish embryos were observed. The expression of AhR-related genes (ahr2, cyp1a1, cyp1b1), heart development key genes (nkx2.5, sox9b), and neurodevelopment key genes (elavl3, gfap, mbp, syn2a) were detected by fluorescence quantitative PCR. [Results]Zebrafish embryos in the BPA exposure group had delayed hatching, decreased heart rate, increased mortality and malformation rate, shortened body length, and decreased movement distance. The differences were statistically significant (P < 0.05). The body length, heart rate and locomotor ability of zebrafish in the CH+BPA group recovered, and the differences were statistically significant (P < 0.05). But the hatching rate, heart rate and mortality rate did not affect. Fluorescence quantitative PCR results showed that AhR-related genes (ahr2, cyp1a1, and cyp1b1) in the BPA group were significantly up-regulated (P < 0.05); key genes for cardiac development (nkx2.5 and sox9b), and genes related to neurodevelopment (elavl3, gfap, mbp, and syn2a) were significantly down-regulated (P < 0.05). The expressions were all down-regulated to varying degrees, and the differences were statistically significant (P < 0.05). The expression of the above genes in the CH+BPA group was significantly improved compared with the BPA exposure group alone, and the difference was statistically significant (P < 0.05). [Conclusion]The activation of AhR signaling pathway is related to the developmental toxicity caused by exposure to BPA in zebrafish embryos.