家蚕DGCR6-Like蛋白基因的序列分析和原核表达
Sequence analysis and prokaryotic expression of DGCR6 protein gene from silkworm, Bombyx mori
从本实验室构建的家蚕cDNA文库中筛选得到一新的cDNA，经生物信息学分析发现其具有DGCR6保守结构域，我们将其命名为家蚕BmDGCR6。家蚕BmDGCR6基因cDNA长为856bp，ORF为534bp，编码长度为177个氨基酸残基的蛋白质。预测该蛋白分子量为20.7kD,等电点6.5。该蛋白与迪格奥尔格综合症相关，先天性免疫缺陷和精神分裂症和该基因也有一定的关联。本实验将BmDGCR6基因克隆至融合表达载体pGEX-4T-3，转化大肠杆菌BL21(DE3)，IPTG诱导表达重组蛋白，并分析了该蛋白的存在形式-包涵体，这为我们进一步研究该蛋白的功能奠定了基础。
novel gene has been obtained from cDNA library of our lab, analysesed with bioinformation and we found it have a critical region on DGCR6, so we name it after BmDGCR6. The full-length of the gene is 856bp. It contains an ORF of 534 bp, predicting it have 177 amino acrids and its molecular weight of protein about 20.737 kD. It’s isoelectric point is 6.5. The protein is a candidate of DGS, also relating to innate immunodeficient disease and schizophrenia. We make the gene of BmDGCR6 into the prokaryotic expression vector pGEX-4T-3, transform into BL21(DE3). The recombinant protein was induced by IPTG, and then identified by SDS-PAGE, the result manifest it was expressed as inclusion body, the fuction of the protein can be studied in future on the stage.
陈剑清、聂作明、朱安平、张耀洲
分子生物学生物工程学昆虫学
家蚕GCR6生物信息学原核表达
Bombyx moriGCR6Bioinformaticsprokaryotic expression
陈剑清,聂作明,朱安平,张耀洲.家蚕DGCR6-Like蛋白基因的序列分析和原核表达[EB/OL].(2009-06-22)[2025-08-05].http://www.paper.edu.cn/releasepaper/content/200906-598.点此复制
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