百草枯对黄孢原毛平革菌产锰过氧化物酶的影响及机制的研究

In order to understand the effect of the reactive oxygen species (Reactive Oxygen Species, ROS) on manganese peroxidase (MnP) effect of white rot fungus, exogenous paraquat was added in this experiment to research on its impact and mechanism of its modes of Phanerochaete Chrysosporium (Phanerochaete Chrysosporium, pc) MnP . A certain concentration of paraquat was added to Phanerochaete Chrysosporium nitrogen enrichment medium, by sampling at different times, to test and analyse the concentration of the biomass, extracellular manganese peroxidase and determination of hydroxyl radical and the possible mechanism of the law and its influence. The results show that, 30 μ mol/L paraquat can significantly improve the activity of Phanerochaete Chrysosporium species (pc530) and its induced mutation (pcR5305) and the activity of MnP, the maximum of which are increased separately by 0.8 and1.6. And pcR5305 are able to resist nutrition and repress its production of MnP. Its activity is much higher than that of pc530. In the first 72h of continuous culture, pcR5305 hydroxyl radical concentration gradually increases, causing the increase in enzyme activity; in the first 96h, pc530 has the same developing trend. The results show that, addition of paraquat produces oxidative stress of Phanerochaete Chrysosporium, hydroxyl radical is a possible mechanism for the enzyme production. Thus, paraquat as exogenous oxidative stress agent, can increase the manganese peroxidase activity, which is beneficial to the degradation of lignin.