athepsin L 在姜黄素诱导的胶质瘤细胞死亡中的作用

im: To investigate the role of cathepsin L on curcumin-induced death in glioma cells.Methods:Cell Counting Kit (CCK)-8 assay, clonogenic survival assay, apoptosis assay ,Western blotting and cell cycle analysis were used to evaluate the effects of curcumin on cell proliferation, apoptosis, and the cell cycle. The expression of cathepsin L ,cyclin B1, bax/ bcl-2 was examined using Western blotting. The expression of cathepsin L also examined by immunocytochemistry. U87 and U251 cells were transfected with cathepsin L siRNA,then treated with curcumin. The apoptotic cells and cell cycle distribution was examined. Results: CCK8 assay and clonogenic survival assay showed that curcumin inhibited U87 and U251 cell proliferation. Flow cytometry analysis showed that curcumin induces apoptosis and cell cycle arrest. Expression of cathepsin L in U87 and U251 cells pretreated with curcumin increased. Cathepsin L siRNA indicated that cathepsin L influenced apoptosis and cell cycle arrest induced by curcumin. Conclusion: Our results suggest that cathepsin L was involved in the apoptosis and cell cycle arrest induced by curcumin. Inhibition of cathepsin L can enhance the sensitivity of glioma cells to curcumin.