酿酒酵母菌中脂滴结构维系因子筛选

Lipid droplets are present in all eukaryotes and some prokaryotes, and are one of the important organelles regulating lipid and energy metabolism in cells.In Saccharomyces cerevisiae, multiple lipid droplets are usually present in the cell, and the droplets are relatively homogeneous in size and stable in structure and number. Even under extreme conditions where large amounts of neutral lipids are rapidly consumed or accumulated in a very short period of time, lipid droplets rarely fuse with each other; however, the structural homeostatic mechanisms by which yeast cells maintain mature lipid droplets are poorly understood. In this study, we screened the surface proteins of lipid droplets of Saccharomyces cerevisiae by oleic acid incubation to search for the role of factors that maintain the structural stability of lipid droplets, and found that Pln1p is essential for the maintenance of relative structural stability of lipid droplets.