9个水稻热激蛋白基因的表达模式
Expression Analysis of Nine Heat Shock Protein Genes in Rice
热休克蛋白（HSPs）广泛存在与生物有机体中。在水稻中，至少存在70个编码 HSPs 的基因，它们中的绝大多数尚未明确功能。本研究利用半定量RT-PCR的方法检测了其中9个尚未明确功能的 OsHSP 基因的表达模式。根据其蛋白分子量，将这九个基因命名为OsHSP80.2, OsHSP74.8, OsHSP71.1, OsHSP58.7, OsHSP50.2, OsHSP26.7, OsHSP24.3, OsHSP24.1 和 OsHSP16.9。研究结果表明，这9个OsHSP 基因在不同的组织中具有不同的表达模式。在不同的胁迫处理条件下，这9个OsHSP 基因的表达模式也是有差异的。9个OsHSP 基因的表达都受到高温的诱导却不受低温的影响；高盐处理下，OsHSP80.2, OsHSP71.1 和 OsHSP24.3 增强表达；10% PEG处理下，OsHSP80.2 和 OsHSP24.1 增强表达；OsHSP71.1 表达被ABA诱导，OsHSP24.1 表达则被ABA抑制。
Heat shock proteins (HSPs) have been found to be ubiquitous. More than 70 putative HSP genes were found in rice (Oryza sativa L.), but few of them have been characterized. Expression patterns of nine rice OsHSP genes were analyzed by semi-quantitative RT-PCR. The nine OsHSPs were named OsHSP80.2, OsHSP74.8, OsHSP71.1, OsHSP58.7, OsHSP50.2, OsHSP26.7, OsHSP24.3, OsHSP24.1 and OsHSP16.9 respectively according to their approximate molecular weight. The nine genes showed different expression profiles in roots, leaves, leaf sheaths, spikes and embryos. All nine OsHSP genes were induced strongly by heat shock treatment, whereas none of them were induced by cold. The transcripts of OsHSP80.2, OsHSP71.1 and OsHSP24.3 were increased during salt tress treatment. Expression of OsHSP80.2 and OsHSP24.1 genes were enhanced while treated with 10% PEG. Only OsHSP71.1 was induced by ABA while OsHSP24.1 was inhibited by ABA.
高国赋、周小云、陈信波、王文芳、邹杰、刘爱玲、张先文
分子生物学植物学遗传学
热激蛋白， 表达模式， 半定量RT-PCR
heat shock proteins (HSPs) expression patterns rice semi-quantitative RT-PCR
高国赋,周小云,陈信波,王文芳,邹杰,刘爱玲,张先文.9个水稻热激蛋白基因的表达模式[EB/OL].(2008-04-16)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/200804-576.点此复制
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