层层组装聚电解质微胶囊的蛋白质吸附和血液相容性
Protein adsorption and blood compatibility of layer-by-layer assembled polyelectrolyte microcapsules
在聚烯丙基胺盐酸盐（PAH）和聚苯乙烯磺酸钠（PSS）通过层层组装制备得到的(PAH/PSS)4/PAH微胶囊最外层通过戊二醛（GA）交联共价接枝聚乙二醇（PEG）或牛血清白蛋白（BSA），或仅通过静电作用吸附一层BSA。以荧光素标记的BSA（FITC-BSA）为血浆模型蛋白，采用荧光显微镜、扫描电子显微镜（SEM）、Zeta-电位仪、流式细胞仪表征各类微胶囊吸附BSA前后的表面形貌及表面电荷的改变。结果表明：未修饰微胶囊与最外层静电吸附上BSA的微胶囊吸附FITC-BSA的量较多，其它各类微胶囊则吸附量较少。最外层接枝PEG和BSA后的微胶囊血浆复钙化时间（PRT）和全血凝固时间（CT）较未修饰的微胶囊均有所延长，且修饰PEG的微胶囊抗凝血性能更好，血液相容性得到改善。
Poly(allyamine hydrochloride) (PAH)/poly(styrene sulfonate) (PSS) microcapsules were fabricated and their surfaces were further modified either physically or chemically with bovine serum albumin (BSA), heparin and polyethylene glycol (PEG). Protein adsorption on the surfaces was investigated by fluorescence microscopy, scanning force microscopy (SEM) and atomic force microscopy (AFM). The amount of BSA adsorbed on the unmodified control microcapsules and the BSA physically modified microcapsules are comparatively larger than that on other capsules. The plasma recalcifation time (PRT) and the whole blood coagulation time (CT) of microcapsules with the chemically bonded BSA and PEG outmost surfaces were prolonged than the unmodified control microcapsules, which indicates the improved blood compatibility.
彭采宇、张裕英、高长有
生物科学研究方法、生物科学研究技术基础医学
层层组装微胶囊血液相容性
layer-by-layer self-assemblymicrocapsulesblood compatibility
彭采宇,张裕英,高长有.层层组装聚电解质微胶囊的蛋白质吸附和血液相容性[EB/OL].(2014-11-20)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201411-342.点此复制
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