N2a细胞不同细胞周期中线粒体动力学相关蛋白的表达变化

Objective: To evaluate the change of mitofusin2 (Mfn2), mitofision1 (Fis1), microtubule-associated protein1 light chain3 (LC3) expression in the different phases of cell cycle of mouse neuroblastoma cells (N2a) in vitro. Methods: double- thymidine block was introduced to synchronize cells in the different phases of cell cycle (G0/G1- phase, S- phase, G2/M-phase) and flow cytometry was used for identification. The expression of Mfn2, Fis1, LC3 were detected by Western-blot. Results: (1) Flow cytometry revealed that cells were synchronized in G0/G1- phase, S- phase and G2/M-phase, respectively. (2) The expression level of Mfn2 and LC3 in S-phase were significantly higher than those in G0/G1 and G2/M-phase. The expression level of Fis1 was different in these three phases, but the difference is not significant. Conclusion: The expression of Mfn2, Fis1, LC3 change with the processing of cell cycle, and the expression levels of Mfn2 and LC3 were higher in S-phase than it in G0/G1 and G2/M-phase.