肺癌肿瘤标志物神经元特异性烯醇化酶抗原适配子的筛选及其应用

Objective To explore a new aptamer-based rapid detection method for lung cancer tumor markers. Methods Highlyspecific adaptive were screened by using systematic evolution of ligands (SELEX) and affinity were detected by flow cytometry.So as to choose a high affinity and high specificity of aptamer. Gold nanoparticles were prepared through sodium citratereduction. The method based on label-free analysis of gold nanoparticles and aptamers were established, with methodologicalinvestigation. Results High specificity and strong affinity of 3 NSE were obtained through 6 rounds of screening, with highestspecificity of a. A rapid method for the detection of NSE antigen were established through the selection of tumor markers oflung cancer and gold nanoparticles. Conclusion The method has a good accuracy for the early detection of lung cancerprovides a new means.