加减青娥方体外对RAW264.7细胞向破骨细胞分化的影响

Objectives: The aim of this study was to explore the effect of Modified Qing E Formula on the osteoclast differentiation from osteoclast precursors RAW264.7 cells. Materials and Methods: The extractions of Modified Qing E Formula with different doses were added into the osteoclast precursors RAW264.7 cultured in vitro. Then the effects of the Modified Qing E Formula on the generation of the RAW264.7 cell,the number and activity of osteoclasts differentiated from osteoclast precursors RAW264.7 induced by receptor activator of nuclear factor κ B factor ligand (RANKL) were detected with tartrate-resistant phosphatase (TRAP) staining. Results: Modified Qing E Formula inhibited the RANKL-induced RAW264.7 cells to differentiate into osteoclasts,same as the action of 17β-estradiol. Conclusions: Modified Qing E Formula could increase bone density to prevent and treat PMOP via suppressing the differentiation and proliferation of osteoclasts.??