新疆雪莲的组织培养及植株再生
In vitro Tissue Culture and Plantlet Regeneration of Saussurea involucrata Kar. et Kir
本文研究了培养基的种类、植物生长调节物质对新疆雪莲愈伤组织诱导、再生苗形成的影响,结果表明:在新疆雪莲愈伤组织诱导过程中,NAA和6-BA组合使用比NAA、6-BA、2,4-D单独使用更有利于愈伤组织的形成, MS培养基对新疆雪莲愈伤组织诱导和生长均最为有利;6,7-V培养基有利于诱导不定芽的形成;生长在1/2MS+ NAA0.5培养基上90%以上的不定芽可在28天内形成不定根。
he combination of NAA and 6-BA was better for the callus induction of Saussurea involucrata than single NAA, 6-BA, and 2,4-D. MS medium was the most effective for callus induction from leaves; 6,7-V medium was the most effective for adventitious buds induction from callus; 90% of the adventitious buds rooted well after they were planted on the 1/2 MS containing 0.5mg.L-1 NAA for 28 days.
戴均贵、杨林
生物工程学植物学
愈伤组织的形成不定芽不定根再生苗新疆雪莲
callus inductionadventitious budsadventitious rootsplantlet regenerationSaussurea involucrata
戴均贵,杨林.新疆雪莲的组织培养及植株再生[EB/OL].(2009-03-27)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200903-1051.点此复制
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