慢病毒介导COX-2基因沉默对Tca8113细胞株成瘤性及侵袭转移能力的影响

Objective To explore the effect of lentivirus-mediated COX-2 on the proliferation and invasion of Tca8113. Methods The frist step was to establish the stable Tca8113 cell lines with silent COX-2. Then to set up lentivirus-mediated COX-2 gene silencing experimental group (pLKO.1shCOX-2), non-carrier negative control group (Tca8113 scr) and normal cells blank control group (Tca8113). Results The western-blot was used to measure the expression of COX-2 protein and the cloning formation assay was used to examine the oncogenicity of Tca8113 impacted by silent lentivirus-mediated COX-2 gene. The changes of Tca8113 in invasion and metastasis were detected by Transwell chamber.Conclusion Down-regulation of COX-2 expression can inhibit the tumorigenicity Tca8113，and it can weaken invasion and etastasis of Tca8113 cells.