不同生长年限华重楼根际土壤微生物多样性研究
为探究华重楼生长发育与土壤微生物群落结构变化的关系，该研究利用 Illumina 高通量测序技术对 3 年生、5 年生、7 年生、9 年生等四个不同生长年限的华重楼根际土壤细菌 16S rRNA 和真菌 18S 序列进行测序分析。结果表明：（1）不同生长年限根际土壤中主要优势细菌均为变形菌门、酸杆菌门、放线菌门和绿弯菌门；优势真菌为子囊菌门、担子菌门与毛霉门。（2）不同生长年限华重楼根际土壤中细菌物种较真菌更丰富，多样化程度更高。细菌多样性随着华重楼生长年限的增加呈降低、升高、再降低的“И”字型规律，最低点在第 5 年、最高点在第 7 年；真菌多样性则随着生长年限的增加呈先升高后下降的“Λ”字型规律，最高点在第 7 年。细菌群落丰富度随着生长年限的增加呈先升高后下降的“Λ”字型规律，第 7 年时丰富度最高；而真菌丰富度随着生长年限增长变化不大。（3）UPGMA聚类分析显示，随着生长年限的推进根际土壤微生物群落结构演变明显，细菌群落演变比较剧烈的时期是在定植后第 7 年、而真菌群落则在定植后第 5 年。（4）Spearman 相关性分析发现速效钾和全氮是影响华重楼根际土壤细菌组成的主要因子，全钾是影响真菌组成的主要因子。综上结果说明不同生长发育时期的华重楼根际土壤微生物群落组成和结构不同，第5~7 年是根际土壤微生物群落多样性产生重大变化的关键时期。
In order to explore the relationship between the growth of Paris polyphylla var.chinensis and the microbial community structure changes of the rhizosphere soil, the Illuminahigh-throughput sequencing technique was used to sequence the 16S rRNA gene of bacteria and18S sequences of fungal at four different growth years, including 3 years, 5 years, 7 years and 9years. The results were as follows: (1) Proteobacteria, Acidobacteria, Actinobacteria andChloroflexi were dominant bacteria in the rhizosphere soil of P. polyphylla in different growthyears. The dominant fungi phylas were Ascomycota, Basidiomycota and Mucoromycota. (2) Thebacterial species in the rhizosphere of Paris polyphylla in different growth years were moreabundant than fungi, and the degree of diversification was higher. The lowest point of bacterialdiversity, which decreased, increased, and then decreased again with the increase of growth yearsshowed a pattern of , was at the 5th year and the highest point was at the 7th year. The fungaldiversity showed a pattern of that first increased and then decreased with the increase ofgrowth years, and the highest point was at the 7th year. The abundance of bacterial communitiesalso showed a pattern of that first increased and then decreased with the increase of growthyears, and the highest point was also at the 7th year. The abundance of fungi did not change muchas the year growing. (3) The UPGMA cluster analysis showed that the microbial communitystructure of the rhizosphere soil had evolved significantly. The period when the bacterialcommunity evolved more drastically was in the 7th year after planting, and the fungal communitywas in the 5th. (4) Spearman correlation analysis found that total nitrogen and available potassiumwere the main factors affecting the composition of rhizosphere soil bacteria, and Total potassiumwas the main factor affecting the composition of fungi. All the above results indicate that thecomposition and structure of the soil microbial community in the rhizosphere of wild P. polyphyllaat different growth and development stages are different, and the 5-7th year is a critical period forsignificant changes in the diverdity of the soil microbial community.
苏江、黄宁珍、何文、付传明、何金祥、谢东斌、刘宝骏、冼康华
微生物学环境生物学生物科学研究方法、生物科学研究技术
华重楼，根际土壤，微生物群落，多样性
苏江,黄宁珍,何文,付传明,何金祥,谢东斌,刘宝骏,冼康华.不同生长年限华重楼根际土壤微生物多样性研究[EB/OL].(2022-03-18)[2025-08-16].https://chinaxiv.org/abs/202203.00062.点此复制
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