不同产地浙贝母生物碱含量及其合成相关基因表达研究

In order to explore the relationship between the accumulation of medicinal components and the expression level of alkaloid synthesis related genes in Fritillaria thunbergii, ultra high performance liquid chromatography mass spectrometryUPLC-MSand real-time quantitative PCRqPCRtechniques were used to determine the total alkaloids (the sum of peimine and peiminine) and the expression level of three genes involved in alkaloid synthesis pathway (HMGR, FPS and DXR) in 11 samples from different producing areas. Meanwhile, biostatistics was used to analyze the correlation between the alkaloid content of mature bulbs and the expression of each gene. The results showed that there were significant differences in total alkaloid content of bulbs at maturity stage (P < 0.05), ranging from 0.2105% to 0.4612%. The variation trend of the expression of HMGR and FPS genes in the tissue of flowering stage and the bulb from flowering stage tomaturity stage is basically consistent with the variation trend of alkaloid content. The expression of DXR gene was the highest in the bulb at maturity stage, and the change trend of the tissue expression at the flowering stage and the bulb expression from the flowering stage to maturity stage was generally inconsistent with the change trend of alkaloid content. The expression levels of HMGR and FPS genes were significantly or extremely significantly positively correlated with peimine, peiminine and total alkaloid content, respectively (P0.05 or P < 0.01). The coefficients between FPS gene expression and alkaloid content were the highest, which were 0.672, 0.631, 0.664, respectively. Meanwhile, the coefficients between DXR gene expression and alkaloid content were low. In summary, the date indicates the accumulation of alkaloids is significantly regulated or modified by HMGR and FPS genes in the MVA pathway, but not by DXR genes in the MEP pathway.