棉花GAUT家族的鉴定与表达分析

otton is the most important fiber cash crop in China. Pectin plays an important role in the growth and development of cotton. Galacturonic acid transferase (Galacturonosyltransferase, GAUT) is a kind of enzymes involved in catalytic glycosylation, also is the key enzyme of pectin on the metabolic pathway, its function has diversity. In this study, based on genomic data of modern cultivated cotton species upland cotton NDM8 and island cotton Pima90, it was found that upland cotton and island cotton had 40 and 41 GAUT family members, respectively. Phylogenetic analysis showed that GAUT family members of cotton and Arabidopsis had homology. Collinearity analysis showed that there were collinearity relationships between most GAUT family members in upland cotton and sea island cotton, in which GhGAUT22 and GbGAUT23 were selected positively, and other members were selected purified. The expression analysis of GAUT family members in upland cotton showed that GAUT family members had temporal and spatial expression characteristics. There were significant differences in response to different stress, among which the response to drought stress was the most obvious. GT-1 element was located in several member promoter regions with significantly up-regulated expression, but it did not exist in multiple member promoters with a large down-regulated trend. qRT-PCR results showed that the expression levels of three genes in upland cotton and sea island cotton were similar to the transcriptome data. In this study, GAUT family members of modern cultivated cotton species were identified from the whole genome level and bioinformatics and expression patterns were analyzed, which provided the basic theoretical basis for studying the molecular biological function of GAUT gene.