针刺调整下丘脑－垂体－卵巢轴异常功能的物质基础研究

In experiemt 1, the push-pull perfusion in hypothalamic preoptic area (MPO) of the ovariectomiezed rats treated with electroacupuncture (name as EA perfusate) was transferred into the MPO of another ovarictomized rat without EA treatment. The blood E2 level in the recipient increased significantly and a few of mature vaginal epithelia reappeared. Those changes were not observed in recipient with perfusate from ovariectomized rat or artifical cerebrospinal fluid. In experiment 2, the push-pull perfusate in hypothalamic preoptic area (MPO) of the rat in different estrus cycle was collected and determined the concentration of neuroactive substances in those perfusate with HPLC and RIA. Then perfusate from rat in pre-estrous cycle (PEP) was microinjected into the MPO of the ovareicetomized rats. The blood E2 level in the ovariectomized rat with PEP microinjection increased significantly compared with the ovariectomized rat with OVXP. A few of mature vaginal epithelia reappeared in ovariecotmized rat with PEP microinjection. Present data suggested that neuroactive substances in MPO were the effective substances of physiological regulation function and pathological changes. They are also the effective substances in electoracupuncture treatment.