鸭茅四倍体与四倍体杂交F2代SSR遗传稳定性分析

For parental cultivars of orchardgrass and fifty F2 progenies of three cross combinations were used in this study. SSR markers were used to analyze genetic relationship of 86 orchardgrass accessions. 15 primer pairs were selected by SSR respectively. 12.5 bands were amplified per primer pairs on average and percent of polymorphic bands was 66.57% in SSR analysis. The conclusion of anayse by clustering tree showed that progenies from the same F2 cross combination clustered together. The data of the genetic distance were agreed with the dendrogram that a stability between the male and female parent and hybrid F2 generation. Each F2 hybrids combinations of genetic variation was relatively stable. Through the SSR appeared to tetraploid and its hybridization F2 generation genetic analysis to determine the genetic stability. As the orchardgrass resource protection, utilization and provide scientific basis for breeding new cultivars.