氯化锰标记前列腺癌细胞株的体外磁共振成像研究

Objective To assess the feasibility and security of prostate cancer cell lines(PC-3) labeled with manganese chloride (MnCl2) for magnetic resonance imaging(MRI) in vitro. Methods The PC-3 that purchased from American Type Culture Collection(ATCC) were recovered, cultured and amplified. The PC-3 were cultured in F-12 HAM'S medium with different concentrations of MnCl2 in cell incubator and collected for MRI after 1 hour. The labeled cells were also collected for MRI in different amount and different time after labeling. The labeled cells were incubated with verapamil 4 hours and the changes of the labeled cellular signal intensities were recorded in different time. Cell Counting Kit-8 (CCK-8) was used to determine the activities of the labeled cells. Results The PC-3 labeled with MnCl2 were high signal intensities on T1-weighted MRI. There were statistically significant differences between labeled cells and no labeled cells (P<0.01). It was the highest signal intensity when PC-3 were labeled with 1.0 mM MnCl2. The signal intensity obviously decreased after 24 hours and becomed to normal signal intensity of unlabeled PC-3 after 72 hours. The PC-3 labeled with 1.0 mM MnCl2 solution showed high signal intensity on T1-weighted MRI with the minimum cell amount of 5.0×105 and lasted to 72 hours after a 4 hours incubation with verapamil. After 4 hours labeling, except the concentration of 0.1 mM, the other concentrations of MnCl2 had a certain toxicity on PC-3 (P<0.05). But, after 24 hours, the concentrations of 0.5-1.0 mM interval had little effect on the viability of PC-3 (P> 0.05). Conclusion The PC-3 could be labeled with MnCl2 and appeared high signal intensity on T1-weighted MRI. The PC-3 were safety labeled with MnCl2 in concentrations which were equal or less than 1.0 mM, but the duration of Mn+2 in PC-3 was shorter. Calcium channel blocker (verapamil) might be extended the duration of PC-3 labeled with MnCl2.