Garcinol对DMBA诱导的地鼠口腔颊囊炎症的抑制作用

Objective:To evaluate the anti-inflammatory effect of Garcinol on DMBA-induced hamster cheek pouch inflammation model. Materials and Methods: The hamsters were divided into five groups. While the negative control group was not treated, the other Syrian hamsters were treated with 0.5% DMBA solution topically to the left cheek pouch three times per week for three consecutive weeks. After the last treatment of DMBA, the hamsters were divided into four groups at random. The positive group was not treated, the other three groups received 0.5mM、5mM、50mM Garcinol respectively three times within the following week. Hamsters were sacrificed at the forth week to obtain tissue samples of the left cheek pouch. They were injected with 5-bromodeoxyuridine (BrdU) 50mg/kg before they were sacrificed . A piece of tissue from each hamster was snap-frozen in liquid nitrogen for PGE2 and LTB4 ELISA analysis. The remaining samples were examined by histopathology and BrdU immunohistochemisty. Results: 1) The number of oral lesions (hyperplasia and dysplasia) decreased after 0.5mM, 5mM, 50mM Garcinol treatment(P<0.05); 2) 0.5mM, 5mM and 50mM Garcinol treatment significantly decreased the number of inflammatory cells/mm2 from 320.51±155.88 to 197.72±83.77, 184.39±64.16,100.41±52.53, respectively(P<0.01); 3) Moderate and high doses of Garcinol decreased the indices of BrdU from 4.26±2.21% to 3.30±1.37% (P<0.05) , 3.01±1.78% (P<0.01) ; 4)With ELISA, we observed that the content of PGE2 was of no significant difference between groups. The LTB4 level of high dose of Garcinol group was significantly decreased compared with positive control(P<0.01). Conclusions: These results indicated that Garcinol have anti-inflammatory, and cell proliferation suppressing effect in DMBA-induced inflammation of hamster cheek pouch.