miR-223通过抑制IGF-1R信号通路诱导人血管平滑肌细胞自噬
MicroRNA-223 induces autophagy of human vascular smooth muscle cells by inhibiting IGF-1R signaling pathway
目的探究miR-223对人动脉平滑肌细胞（HASMCs）自噬功能调控的分子机制。方法收集正常捐献者的下肢动脉标本并提取HASMCs，通过RT-qPCR、免疫印迹反应实验观察比较实验组（转染miR-223 mimic）和阴性对照组IGF-1R-PI3K/Akt信号通路表达差异以及自噬相关蛋白表达差异。结果转染miR-223 mimic的HASMCs与阴性对照组相比，IGF-1R-PI3K/Akt信号通路明显下调，Western blotting结果表明自噬功能明显增强。结论 miR-223在HASMCs中通过与靶基因IGF-1R mRNA的3\'-UTR直接结合，降解mRNA并抑制下游PI3K/Akt信号传导通路的激活，导致HASMCs细胞自噬水平的明显升高。?????
Purpose To explore the molecular mechanism of miR-223 on the autophagy function of human artery smooth muscle cells (HASMCs) Methods Donors\' normal lower limb arteries were collected and HASMCs were extracted. By RT-qPCR and western blot, differences of IGF-1R-PI3K/Akt signaling pathway expression and autophagy related protein expression between experimental group (miR-223 mimic transfected) and negative control group were observed. Results HASMCs transfected with miR-223 mimic showed a significant down-regulation in the IGF-1R-PI3K/Akt signaling pathway compared with the negative control group, with significant enhancement of autophagy flux. Conclusion miR-223 binds IGF-1R mRNA 3 \'UTR in HASMCs, which leads to degradation of the mRNA and suppression of the activation of IGF-1R-PI3K/Akt signaling pathway, and up-regulates HASMCs\' autophagy flux.
刘陈枢、李梓伦、吴伟滨、王深明、常光其
基础医学分子生物学生理学
分子生物学miR-223自噬IGF-1R信号通路。
molecular biologymicroRNA-223autophagyIGF-1R signaling pathway
刘陈枢,李梓伦,吴伟滨,王深明,常光其.miR-223通过抑制IGF-1R信号通路诱导人血管平滑肌细胞自噬[EB/OL].(2017-12-15)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201712-206.点此复制
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