酪氨酸及其某些重要衍生物的毛细管电泳分离与检测
Separation and determination of L-Tyrosine and its metabolites by capillary electrophoresis with UV detection
采用毛细管电泳紫外检测法（CE-UV），对酪氨酸及其某些重要衍生物酪胺、对羟基苯丙酮酸、尿黑酸以及含酪氨酸残基的活性肽Tyr-Arg, Tyr-D-Arg 和Tyr-Gly-Gly等七种生物物质进行了分离分析研究，考察了运行缓冲溶液的pH与浓度，分离电压，进样时间,不同添加剂及其浓度等对CZE分离的影响，在优化条件下七组分在20 min以内达到完全基线分离。并将该优化方法直接用于尿样中七种组分回收率及精密度的测定，结果令人满意。
method of capillary electrophoresis with UV detection has been developed for separation and determination of L-tyrosine (Tyr) and its metabolites, such as Tyramine (TA), p-hydroxyphenylpyruvic (pHPP), Homogentisic acid (HGA) and some dipeptides containing Tyr, such as Tyr-Gly-Gly (YGG), Tyr-Arg (YR) and Tyr-D-Arg (Y-D-R). The conditions for separation of these compounds were optimized in detail. Under the optimum conditions, the seven compounds were completely separated within 20 min. The proposed method has been applied to determine the seven compounds in the spiked human urine sample.
石焱芳、陈国南
生物化学生物科学研究方法、生物科学研究技术
毛细管电泳，紫外检测，酪氨酸及其某些重要衍生物
capillary electrophoresisUV detectionL-Tyrosine and its metabolites
石焱芳,陈国南.酪氨酸及其某些重要衍生物的毛细管电泳分离与检测[EB/OL].(2008-03-19)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/200803-505.点此复制
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