联合应用磁性纳米Fe3O4颗粒和5溴汉防己甲素对柔红霉素诱导的K562/A02细胞凋亡效应的影响
Effects of Magnetic Nanoparticle of Fe3O4 and 5Bromotetrandrin on apoptosis induced by Daunorubicin in K562/A02 Leukemic cells
本研究探讨磁性纳米Fe3O4颗粒和5溴汉防己甲素联合治疗慢性髓系白血病的潜在可能。采用流式细胞术和Wright染色检测细胞凋亡情况;采用Western blot法检测细胞BCL-2和BAX表达水平。结果表明：MNP(Fe3O4) 或5-BrTet 结合DNR后对K562/A02细胞有细胞毒作用，而联合应用MNP(Fe3O4)和5-BrTet可协同促进DNR诱导K562/A02细胞凋亡,且细胞出现典型的凋亡形态改变,同时可见BCL-2表达下调,BAX表达上调。结论:MNP(Fe3O4)或5-BrTet结合DNR后均能有效促进K562/A02细胞凋亡，两者联合应用作用增强，其机制可能与BCL-2表达下调及BAX表达上调有关。
he aim of this study was to investigate the potential benefit of combination therapy with Magnetic Nanoparticle of Fe3O4 and 5Bromotetrandrine on chronic leukemia. We detected the apoptosis by flow cytometry(FCM) and Wright staining. The expression of BAX and BCL-2 was measured by Western blot.The results showed that combination of DNR with either MNP(Fe3O4) or 5-BrTet exerted a potent cytotoxic effect on K562/A02 cells, while MNP(Fe3O4) and 5-BrTet cotreatment could synergistically enhance DNR-induced apoptosis. After treated with this composite, K562/A02 cells appeared typical apoptotic morphological features; the expression level of BCL-2 decreased and BAX increased markedly.It is concluded that MNP(Fe3O4) or 5-BrTet with DNR induced apoptosis in K562/ A02 cells, and when used together，they had distinct synergism .The down-regulation of BCL-2 and the up-regulation of BAX may play important roles.
刘莉洁、陈宝安、沈明芳、高峰、王雪梅、李小毛、孙新臣、丁家华、陈文姬、高冲、李国宏、许文林、程坚
基础医学肿瘤学细胞生物学
K562 /A02白血病细胞多药耐药磁性纳米Fe3O4颗粒5溴汉防己甲素凋亡BCL-2BAX
K562/A02 leukemic cellsmultidrug resistanceMagnetic Nanoparticle of Fe3O45BromotetrandrineapoptosisBCL-2BAX
刘莉洁,陈宝安,沈明芳,高峰,王雪梅,李小毛,孙新臣,丁家华,陈文姬,高冲,李国宏,许文林,程坚.联合应用磁性纳米Fe3O4颗粒和5溴汉防己甲素对柔红霉素诱导的K562/A02细胞凋亡效应的影响[EB/OL].(2009-01-23)[2025-07-16].http://www.paper.edu.cn/releasepaper/content/200901-1126.点此复制
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