马尾松胚性细胞悬浮增殖培养体系的建立

Suspension cultures were firstly established with embryogenic tissues initiated from immature zygotic embryos in Pinus massoniana Lamb. (masson pine ). The effects of initial tissues weight, cell density in subculture , the dynamic changes of cell growth and cell viability were investigated through measuring the sediment cell volume (SCV) and cell viability by spectrophotometer during establishment of suspension cultures. The results showed that: the growth of embryogenic tissues was significantly influenced by initial tissues weight and cells density in suspension cultures; the "S" cells growth kinetics were shown during 20 days suspension culture period, and the cells viability sharply increased at first and gradually decreased in later.A better suspension culture system in masson pine was established: 1.0g tissues per 30ml fresh liquid media was the appropriate amount for the suspension culture of embryogenic cells and the mean growth rate could reach to 12.097; when cell density was 1/6 in volume in subculture, the cells showed more proliferation and better cell state; the appropriate subculture period was identified as 8-12 days. This study provided a basic information for investigating growth kinetic of suspension cultures and optimization of mass propagation system in masson pine somatic embryogenesis. It is also benefit to the genetic transformation and cell fusion in masson pine breeding improvement.