Visible Thrombolysis Acceleration of a Nanomachine Powered by Light-Driving F0F1-ATPase Motor
We report on thrombolysis acceleration of a nanomachine powered by light-driving delta-subunit-free F0F1-ATPase motor. It is composed of a mechanical device, locating device, energy storage device, and propeller. The rotory delta-subunit-free F0F1-ATPase motor acts as a mechanical device, which was obtained by reconstructing an original chromatophore extracted from Rhodospirillum rubrum. We found that the bioactivity of the F0F1-ATPase motor improved greatly after reconstruction. The zeta potential of the nanomachine is about -23.4 mV. Cytotoxicity induced by the nanomachine was measured using cell counting kit (CCK)-8 assay. The A549 cells incubated with different fractional concentrations of the nanomachine within 48 h did not show obvious cytotoxicity. The locating device helps the nanomachine bind to the thrombi. Energy was easily stored by exposing the nanomachine to 600-nm-wavelength irradiation, which promoted activity of the motor. The rotation of the long propeller accelerated thrombolysis of a blood clot in vitro in the presence of urokinase (UK). This result was based on visual inspection and confirmed by a series of tests.
We report on thrombolysis acceleration of a nanomachine powered by light-driving delta-subunit-free F0F1-ATPase motor. It is composed of a mechanical device, locating device, energy storage device, and propeller. The rotory delta-subunit-free F0F1-ATPase motor acts as a mechanical device, which was obtained by reconstructing an original chromatophore extracted from Rhodospirillum rubrum. We found that the bioactivity of the F0F1-ATPase motor improved greatly after reconstruction. The zeta potential of the nanomachine is about -23.4 mV. Cytotoxicity induced by the nanomachine was measured using cell counting kit (CCK)-8 assay. The A549 cells incubated with different fractional concentrations of the nanomachine within 48 h did not show obvious cytotoxicity. The locating device helps the nanomachine bind to the thrombi. Energy was easily stored by exposing the nanomachine to 600-nm-wavelength irradiation, which promoted activity of the motor. The rotation of the long propeller accelerated thrombolysis of a blood clot in vitro in the presence of urokinase (UK). This result was based on visual inspection and confirmed by a series of tests.
Yue, Jiachang、Liu, Lifeng、Liu, Lifeng、Duan, Xiaoxia、Jiang, Weijian、Liu, Lifeng、Jiang, Weijian
生物工程学生物物理学基础医学
UTE ISCHEMIC-STROKEISSUE-PLASMINOGEN ACTIVATORHITOSAN NANOPARTICLESLINICAL-PRACTICERUG-DELIVERYHEART-DISEASEHERAPYSSOCIATIONMARKERSHEMORRHAGE
Yue, Jiachang,Liu, Lifeng,Liu, Lifeng,Duan, Xiaoxia,Jiang, Weijian,Liu, Lifeng,Jiang, Weijian.Visible Thrombolysis Acceleration of a Nanomachine Powered by Light-Driving F0F1-ATPase Motor[EB/OL].(2016-05-12)[2025-05-15].https://chinaxiv.org/abs/201605.01528.点此复制
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