BX3促进干细胞向神经元分化的研究

Objective: To investigate the effect of CBX3 on the differentiation of mouse embryonic stem cells. Methods: CBX3 was transfected into mouse ES-D3 cells, extracted mRNA and protein of ES-D3 cells, then detected the mRNA and protein level of Nestin, Sox2, Gfap and TUBB3 by real time quantitative PCR and Western-blot.And CBX3 specific siRNA was transfected into differentiated mouse ESCs. Control siRNA was used as negative control group. After transfecting into mouse ES-D3 cells, the mRNA and protein lelvel of Nestin,Sox2,Gfap and TUBB3 were detected. Results: The mRNA and protein expression of Nestin and Sox2 of transfected ESCs cells were decreased after overexpression of CBX3.But The mRNA and protein expression of Gfap and TUBB3 were increase（P<0.05）. After interferencing CBX3 expression by siRNA, It was found that the mRNA and protein expression level of Nestin and Sox2 maintained a high level, but mRNA and protein expression level of the differentiation markers (Gfap and TUBB3) were low contrast to the non transfected cells (P<0.05). Conclusion: CBX3 has the ability to promote the differentiation of embryonic stem cells.